Goat Anti-PIWI / HIWI Antibody

$464.00

Category: Primary Antibodies
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SKU: EB06677
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
piwi homolog; piwi-like 1|MIWI|homolog of Drosophila piwi|piwi (Drosophila)-like 1|piwi-like 1 (Drosophila)|PIWI|HIWI|PIWIL1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_004755.2; NP_001177900.1
Blocking Peptide
EBP06677
Immunogen
Peptide with sequence C-SNRKDKYDAIKKY, from the internal region of the protein sequence according to NP_004755.2; NP_001177900.1.
Product Comments
This antibody is expected to recognise both reported isoforms (NP_004755.2; NP_001177900.1).
Peptide Sequence
C-SNRKDKYDAIKKY
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human
Human Gene ID
9271
Mouse Gene ID
57749
Product Grade
IHC Results
In paraffin embedded Human Testis shows textured cytoplasm staining in all germ cells. Recommended concentration: 4-6µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Application Type
Pep-ELISA, IF, IHC, FC

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