Goat Anti-CD32 / FCGR2B Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

Fc fragment of IgG, low affinity IIb, receptor|CD32B|Fc fragment of IgG, low affinity IIb, receptor (CD32)|Fc fragment of IgG, low affinity II, receptor for (CD32)|Fc gamma RIIb|Fc fragment of IgG, low affinity IIb, receptor for (CD32)|IGFR2|FCGR2|FCG2|CD32|FCGR2B

Immunofluorescence: Strong expression of the protein seen in the membranes of THP-1 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

NP_003992.3; NP_001002273.1; NP_001002274.1; NP_001002275.1; NP_001177757.1

EBP06695

Peptide with sequence C-PDALEEPDDQNRI, from the C Terminus of the protein sequence according to NP_003992.3; NP_001002273.1; NP_001002274.1; NP_001002275.1; NP_001177757.1.

This antibody is expected to recognise all reported isoforms (NP_001002273.1; NP_001002274.1; NP_001002275.1; NP_003992.3; NP_001177757.1).

C-PDALEEPDDQNRI

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

Human

2213

Paraffin embedded Human Placenta. Recommended concentration: 6-8µg/ml.

Antibody detection limit dilution 1:32000.

Approx. 38kDa band observed in Human Placenta lysates and in lysates of cell line Daudi, and also in preliminary testing of K562 cell lysate (calculated MW of 34.0kDa according to NP_003992.3). This molecular weight is routinely observed by other sources. Recommended concentration: 0.5-3µg/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, FC, IF, IHC