Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names agouti related protein homolog|MGC118963|Agouti-related transcript, mouse, homolog of|agouti (mouse) related protein|agouti related protein homolog (mouse)|ASIP2|AGRT|ART|AGRP | |
Accession ID NP_001129.1; NP_015531.1 | |
Blocking Peptide EBP06725 | |
Immunogen Peptide with sequence CRKLGTAMNPCSRT, from the C Terminus of the protein sequence according to NP_001129.1; NP_015531.1. | |
Product Comments This antibody is expected to recognise both reported isoforms (as represented by NP_001129; NP_015531) | |
Peptide Sequence CRKLGTAMNPCSRT | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Dog, Pig | |
Reactive Species Human | |
Human Gene ID 181 | |
Product Grade  | |
IHC Results Frozen section of Human Hypothalamus shows staining of a group of neuronal cell bodies in the human infundibular (arcuate) nucleus and their varicose axons. Recommended concentration: 0.05-0.1µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Preliminary experiments gave bands at approx 45kDa and 24kDa in Human Brain lysates after 0.1µg/ml antibody staining. Thiese bands were successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please note that we cannot currently find an explanation in the literature given the calculated size of 14.4kDa according to NP_001129.1and NP_015531.1. | |
Application Type Pep-ELISA, IHC |
Goat Anti-AGRP / Agouti-related protein Antibody
$423.00
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Selected References [{"pmid": 25084101, "intro": "This antibody has been successfully used as a specificity control in the following paper:", "title": "Colocalization of Cocaine- and Amphetamine-Regulated Transcript with Kisspeptin and Neurokinin B in the Human Infundibular Region", "author": "Katalin Skrapits, Beáta Á Borsay, László Herczeg, Philippe Ciofi, Stephen R Bloom, Mohammad A Ghatei, Waljit S Dhillo, Zsolt Liposits, Erik Hrabovszky", "journal": "PLoS One. 2014 Aug 1;9(8):e103977."}] |