Goat Anti-MAPK3/ERK1 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB06751
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
extracellular signal-related kinase 1|extracellular signal-regulated kinase 1|OTTHUMP00000174538|MGC20180|HUMKER1A|HS44KDAP|protein kinase, mitogen-activated 3 (MAP kinase 3 p44)|PRKM3|P44MAPK|P44ERK1|HGNC:6877|mitogen-activated protein kinase 3|ERK1|MAPK3
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_002737.2; NP_001035145.1; NP_001103361.1
Blocking Peptide
EBP06751
Immunogen
Peptide with sequence GGEPRRTEGVGP-C, from the N Terminus of the protein sequence according to NP_002737.2; NP_001035145.1; NP_001103361.1.
Product Comments
No cross-reactivity expected with ERK2
Peptide Sequence
GGEPRRTEGVGP-C
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
5595
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:4000.
Western Blot
Approx 40kDa band observed in Human Cerebellum lysates and in lysates of cell lines A431 and HepG2, and approx 42kDa obseved in Human Frontal Cortex lysates (calculated MW of 40.1kDa according to NP_001035145.1 and 43.1kDa according to NP_002737.2). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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