Goat Anti-Neuroligin 1 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB06865
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
NLGN1|KIAA1070|MGC45115|neuroligin 1|OTTHUMP00000211275|OTTHUMP00000211276
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the membranes/ cytoplasm of U251 and U2OS cells. Recommended concentration: 10µg/ml.
Accession ID
NP_055747.1
Blocking Peptide
EBP06865
Immunogen
Peptide with sequence C-TAKQDDPKQQ, from the internal region of the protein sequence according to NP_055747.1.
Peptide Sequence
C-TAKQDDPKQQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
22871
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:4000.
Western Blot
Preliminary experiments gave an approx 150kDa band in Human Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.0 kDa according to NP_055747.1. The 150kDa band was successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA, IF

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