Goat Anti-Karyopherin (importin) beta 1 Antibody

$464.00

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SKU: EB07018
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
IPO1|KapB1|nuclear factor p97|karyopherin beta 1|importin beta-1 subunit|importin 90|NTF97|MGC2157|MGC2156|MGC2155|Impnb|IPOB|IMB1|KPNB1|HGNC:6400|karyopherin (importin) beta 1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_002256.2; NP_001263382.1
Blocking Peptide
EBP07018
Immunogen
Peptide with sequence C-RRSKTNKAKTLAT, from the C Terminus of the protein sequence according to NP_002256.2; NP_001263382.1.
Peptide Sequence
C-RRSKTNKAKTLAT
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human, Rat
Human Gene ID
3837
Mouse Gene ID
16211
Rat Gene ID
24917
Product Grade
IHC Results
Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 90-95-kDa band observed in lysates of cell lines A431, Daudi, HEK293, HeLa, Jurkat, Kelly and KNRK (calculated MW of 97.2kDa according to Human NP_002256.2 and Rat NP_058759.2). Recommended concentration: 0.01-0.1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, IF, FC