Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names phosphodiesterase 4D|FLJ97311|DKFZp686M11213|phosphodiesterase 4D, cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E3)|dunce-like phosphodiesterase E3|cAMP-specific phosphodiesterase PDE4D6|cAMP-specific phosphodiesterase 4D|STRK1|PDE4DN2|HSPDE4D|DPDE3|HGNC:8783|phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)|PDE4D | |
Usage Summary ELISA: This antibody has been successfully used in ELISA as described in the following paper: Bazhin et al, Mol Immunol. 2010 Jan;47(4):678-84.; PMID: 19939455. | |
Accession ID NP_001184151.1; NP_001184150.1; NP_006194.2; NP_001098101.1; NP_001184152.1; NP_001159371.1 | |
Blocking Peptide EBP07046 | |
Immunogen Peptide with sequence QPEACVIDDRSPDT, from the C Terminus of the protein sequence according to NP_001184151.1; NP_001184150.1; NP_006194.2; NP_001098101.1; NP_001184152.1; NP_001159371.1. | |
Product Comments This antiobody is expected to recognize all reported isoforms. | |
Peptide Sequence QPEACVIDDRSPDT | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 5144 | |
Product Grade  | |
IHC Results Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 5µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx 70+80+90kDa bands observed in lysates of cell line A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approx. 68kDa and 95kDa are observed. These bands are not observed in the untransfected COS1. Calculated MW of 91.1kDa according to PDE4D1 (NP_001098101.1), 76.5kDa according to PDE4D2 (NP_006194.2) and 84.7kDa according to PDE4D3 (NP_001159371.1). Recommended concentration: 0.1-0.3µg/ml. | |
Application Type Pep-ELISA, WB-Trf, IHC, EIA |
Goat Anti-PDE4D Antibody
$464.00
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Selected References [{"pmid": 19939455, "intro": "This antibody has been successfully used in ELISA in the following paper:", "title": "Distinct metabolism of cyclic adenosine monophosphate in regulatory and helper CD4+ T cells.", "author": "Bazhin AV, Kahnert S, Kimpfler S, Schadendorf D, Umansky V.", "journal": "Mol Immunol. 2010 Jan;47(4):678-84."}] |