Goat Anti-Engrailed-2 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB07120
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
AUTS1|EN2|engrailed homolog 2|Engrailed-2
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of HEK293 and U2OS cells. Recommended concentration: 10µg/ml.
Accession ID
NP_001418.2
Blocking Peptide
EBP07120
Immunogen
Peptide with sequence C-NHSTTAKEGKSDSE, from the C Terminus of the protein sequence according to NP_001418.2.
Peptide Sequence
C-NHSTTAKEGKSDSE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human, Mouse
Human Gene ID
2020
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:4000.
Western Blot
Approx 35kDa band observed in Mouse Brain lysates (calculated MW of f 33.8kDa according to Mouse NP_034264.1). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF

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