Goat Anti-CXCR3 / GPR9 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

chemokine (C-X-C) receptor 3|Mig receptor|IP10 receptor|G protein-coupled receptor 9|MigR|Mig-R|IP10-R|IP10|GPR9|CMKAR3|CKR-L2|CD183|HGNC:4540|chemokine (C-X-C motif) receptor 3|CXCR3

Immunofluorescence: Strong expression of the protein seen in the membranes of A431 and HepG2 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

NP_001495.1; NP_001136269.1

EBP07201

Peptide with sequence C-RRDSSWSETSEA, from the C Terminus of the protein sequence according to NP_001495.1; NP_001136269.1.

C-RRDSSWSETSEA

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat

Human

2833

12766

84475

Paraffin embedded Human Spleen. Recommended concentration: 6-8µg/ml.

Antibody detection limit dilution 1:64000.

Preliminary experiments showed a band at approx 55-60kDa in Human Tonsil and Mouse Spleen lysates after 1µg/ml antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please note we cannot currently find an explanation in the literature for this band, given the calculated size of 45.5kDa according to NP_001136269.1

Pep-ELISA, IF, IHC, FC