Goat Anti-XRCC4-like factor / NHEJ1 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB07440
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
Cernunnos|XLF|FLJ12610|nonhomologous end-joining factor 1|XRCC4-like factor|NHEJ1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in HepG2 and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_079058.1; NP_001364428.1
Blocking Peptide
EBP07440
Immunogen
Peptide with sequence C-QRPQLSKVKRKKPR, from the C Terminus of the protein sequence according to NP_079058.1; NP_001364428.1.
Peptide Sequence
C-QRPQLSKVKRKKPR
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
79840
Product Grade
IHC Results
In paraffin embedded Human Placenta shows nuclear staining in trophoblasts. Recommended concentration: 2.5-3.8µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx. 38kDa band observed in nuclear lysates of cell lines HeLa, A431, Jurkat and K562, and approx. 37kDa in Human Skeletal muscle and in nuclear lysates of cell line HepG2 (calculated size of 33.3kDa according to NP_079058.1). These molecular weights have been observed by other sources. Recommended concentration 0.03-0.1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, IHC, FC

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