Goat Anti-ICAM5 precursor Antibody

$423.00

Category: Primary Antibodies
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SKU: EB07506
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
ICAM5|intercellular adhesion molecule 5, telencephalin|TLCN|TLN|intercellular adhesion molecule 5|telencephalin
Accession ID
NP_003250.3
Blocking Peptide
EBP07506
Immunogen
Peptide with sequence C-ESPAEGEVFAIQ, from the C Terminus of the protein sequence according to NP_003250.3.
Peptide Sequence
C-ESPAEGEVFAIQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Pig, Cow
Reactive Species
Human
Human Gene ID
7087
Mouse Gene ID
15898
Rat Gene ID
313785
Product Grade
IHC Results
In paraffin embedded Human Brain (Cerebral Cortex) shows axon/dendrite staining leaving cell bodies relatively unstained. Recommended concentration, 2-4µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Preliminary experiments gave an approx 200-250kDa band in lysates of Human Brain (Frontal Cortex and Hippocampus) and Mouse Brain after 0.05 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 97.2kDa according to NP_003250.2. The 200-250kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA, IHC

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