Goat Anti-S3-12 / KIAA1881 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB07731
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
hCG1646516|plasma membrane associated protein, S3-12|S3-12|KIAA1881
Accession ID
NP_001073869.1
Blocking Peptide
EBP07731
Immunogen
Peptide with sequence C-RTTDNGGEQTALSPQE, from the internal region of the protein sequence according to NP_001073869.1.
Peptide Sequence
C-RTTDNGGEQTALSPQE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
729359
Product Grade
IHC Results
In paraffin embedded Human Pancreas shows membranous and cytoplasm staining in adipocytes. Recommended concentration, 3-6µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:2000.
Western Blot
Preliminary experiments gave bands at approx 45kDa and 50kDa in Human Adipose lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 134kDa according to NP_001073869.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Application Type
Pep-ELISA, IHC

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