Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size 100 µg
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names pancreas transcription factor 1 subunit alpha\|p48 DNA-binding subunit of transcription factor PTF1\|exocrine pancreas-specific transcription factor p48\|class II bHLH protein PTF1A\|bHLH transcription factor p48\|pancreas specific transcription factor, 1a\|PTF1-p48\|PTF1A
Accession ID NP_835455.1
Blocking Peptide EBP07839
Immunogen Peptide with sequence C-WTDEKQLKEQN, from the internal region of the protein sequence according to NP_835455.1.
Peptide Sequence C-WTDEKQLKEQN
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions Refrigerated
Predicted Species Human, Mouse, Rat, Dog, Zebrafish
Reactive Species Human, Mouse
Human Gene ID 256297
Mouse Gene ID 19213
Rat Gene ID 117034
Product Grade
ELISA Detection Limit Antibody detection limit dilution 1:128000.
Western Blot Approx 37kDa observe in Human Pancreas lysates and approx.38-40kDa in lysates of cell lines Jurkat, U2OS and NIH3T3 ,(calculated MW of 35.0kDa according to Human NP_835455.1 and 35.2kDa according to Mouse NP_061279.2). Recommended concentration: 0.5-2µg/ml. Primary incubation 1 hour at room temperature.
Application Type Pep-ELISA, WB

Selected References

[{"pmid": 19056911, "intro": "This antibody (previous batch) has been successfully used in ICC in Human and Mouse:", "title": "Biphasic Induction of Pdx1 in Mouse and Human Embryonic Stem Cells Can Mimic Development of Pancreatic ?-Cells.", "author": "Andreia S. Bernardo, Candy H.-H. Cho, Sharon Mason, Hilary M Docherty, Roger A. Pedersen, Ludovic Vallier and Kevin Docherty.", "journal": "Stem Cells. 2009 Feb;27(2):341-51."}, {"pmid": 21852532, "intro": "This antibody (previous batch) has been successfully used in Western blot in Mouse:", "title": "LRH-1 and PTF1-L coregulate an exocrine pancreas-specific transcriptional network for digestive function.", "author": "Holmstrom SR, Deering T, Swift GH, Poelwijk FJ, Mangelsdorf DJ, Kliewer SA, MacDonald RJ.", "journal": "Genes Dev. 2011 Aug 15;25(16):1674-9."}]

Goat Anti-PTF1A / PFT1-P48 Antibody

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