Goat Anti-Insulysin / Insulinase Antibody

$423.00

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SKU: EB07898
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
insulinase|insulysin|insulin-degrading enzyme|FLJ35968|INSULYSIN|IDE
Accession ID
NP_004960.2; NP_001159418.1; NP_001309722.1; NP_001309723.1; NP_001309724.1; NP_001309726.1
Blocking Peptide
EBP07898
Immunogen
Peptide with sequence C-QQYNFDRDNTE, from the internal region of the protein sequence according to NP_004960.2; NP_001159418.1; NP_001309722.1; NP_001309723.1; NP_001309724.1; NP_001309726.1.
Product Comments
This antibody is expected to recognize all reported isoforms (NP_004960.2; NP_001159418.1; NP_001309722.1; NP_001309723.1; NP_001309724.1; NP_001309726.1). Reported variants represent identical protein: NP_001309725.1, NP_001309724.1
Peptide Sequence
C-QQYNFDRDNTE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Dog
Reactive Species
Human
Human Gene ID
3416
Product Grade
IHC Results
Paraffin embedded Human Lung and Breast. Recommended concentration: 2.5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 110-120kDa band observed in lysates of cell line K562 (calculated MW of 118kDa according to NP_004960.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC