Goat Anti-Tcfec (mouse) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

TFEC|MGC107474|BB107417|transcription factor EC|Tcfec

NP_112475.1

EBP07934

Peptide with sequence C-HITKQQTHPERN, from the internal region of the protein sequence according to NP_112475.1.

C-HITKQQTHPERN

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Mouse, Rat

Mouse, Human

21426

26296

In paraffin embedded Mouse Thymus shows nuclear staining in sporadic cells of the medula. Recommended concentration: 4-6µg/ml. Paraffin embedded Human Kidney and Spleen. Recommended concentration: 5µg/ml.

Antibody detection limit dilution 1:32000.

Preliminary experiments gave one band at approx 37kDa and much stronger bands at approx 70+28kDa in Mouse Testis lysates after 0.5 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the stonger bands we observe given the calculated size of 35.1kDa according to Mouse NP_112475.1. All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Pep-ELISA, IHC