Goat Anti-CLEC16A Antibody

$423.00

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SKU: EB08076
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
MGC111457|KIAA0350|Gop-1|C-type lectin domain family 16, member A|CLEC16A
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei and weak expression seen in the cytoplasm of A549 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_056041.1; NP_001230332.1
Blocking Peptide
EBP08076
Immunogen
Peptide with sequence C-SLENQDKGGERP, from the internal region of the protein sequence according to NP_056041.1; NP_001230332.1.
Peptide Sequence
C-SLENQDKGGERP
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat
Reactive Species
Human
Human Gene ID
23274
Mouse Gene ID
74374
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Preliminary experiments gave an approx 70kDa band in Rat Testis lysates after 0.5µg/ml antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please note that we currently cannot find an explanation in the literature for this band, given the calculated size of 115kDa according to Rat XP_213209.4.
Application Type
Pep-ELISA, IF, FC