Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names phorbolin 1|bK150C2.1|PHRBN|ARP3|apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A|APOBEC3A | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and A549 cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_663745.1 | |
Blocking Peptide EBP08268 | |
Immunogen Peptide with sequence C-TSNFNNGIGRHKTY, from the internal region of the protein sequence according to NP_663745.1. | |
Peptide Sequence C-TSNFNNGIGRHKTY | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 200315 | |
Product Grade ![]() | |
IHC Results In paraffin embedded Human Tonsil shows staining of distinct parts of the nucleus in lymphoid cells. Recommended concentration: 3-6µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:8000. | |
Western Blot Approx. 26-28kDa band observed in Human Spleen, Tonsil and Peripheral Blood Monocytes (PBM) lysates (calculated MW of 23.0kDa according to Human NP_663745.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-1ug/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IF, FC, IHC |
Goat Anti-Phorbolin 1 / APOBEC3A Antibody
$464.00
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