Goat Anti-Phorbolin 1 / APOBEC3A Antibody

$464.00

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SKU: EB08268
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
phorbolin 1|bK150C2.1|PHRBN|ARP3|apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A|APOBEC3A
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and A549 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Accession ID
NP_663745.1
Blocking Peptide
EBP08268
Immunogen
Peptide with sequence C-TSNFNNGIGRHKTY, from the internal region of the protein sequence according to NP_663745.1.
Peptide Sequence
C-TSNFNNGIGRHKTY
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
200315
Product Grade
IHC Results
In paraffin embedded Human Tonsil shows staining of distinct parts of the nucleus in lymphoid cells. Recommended concentration: 3-6µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Approx. 26-28kDa band observed in Human Spleen, Tonsil and Peripheral Blood Monocytes (PBM) lysates (calculated MW of 23.0kDa according to Human NP_663745.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-1ug/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC, IHC