Goat Anti-GADD45gamma (aa 18 to 28) Antibody

$423.00

Category: Primary Antibodies
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SKU: EB08299
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
growth arrest and DNA-damage-inducible gamma|gadd-related protein, 17 kD|GADD45-gamma|GRP17|GADD45gamma|DDIT2|CR6|growth arrest and DNA-damage-inducible, gamma
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and A549 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_006696.1
Blocking Peptide
EBP08299
Immunogen
Peptide with sequence C-RMQGAGKALHE, from the internal region of the protein sequence according to NP_006696.1.
Peptide Sequence
C-RMQGAGKALHE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Cow
Reactive Species
Human
Human Gene ID
10912
Mouse Gene ID
23882
Rat Gene ID
291005
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary testing showed a band at approx 23kDa in lysate of cell line A549 and in Human Prostate and Testes lysate at a concentration of 0.5-1ug/ml (calculated Mwt.of 17.1kDa according to NP_006696.1). This molecular weight is observed by other sources. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IF, FC

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