Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names HRASLS2|HRAS-like suppressor 2|FLJ20556 | |
Accession ID NP_060348.1 | |
Blocking Peptide EBP08620 | |
Immunogen Peptide with sequence QELPYSLTSDNC, from the internal region of the protein sequence according to NP_060348.1. | |
Product Comments This design was based on provisional sequence data. | |
Peptide Sequence QELPYSLTSDNC | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 54979 | |
Product Grade  | |
IHC Results In paraffin embedded Human Liver shows a granular pattern in the cytoplasm of hepatocytes. Recommended concentration, 5-10µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:16000. | |
Western Blot Preliminary experiments gave an approx. 21kDa band in Human Kidney lysates after 0.5 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.4kDa according to NP_060348.1 (provisional). The 21kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? | |
Application Type Pep-ELISA, IHC |
Goat Anti-HRASLS2 Antibody
$423.00
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