Goat Anti-Monoglyceride Lipase Antibody

$464.00

Category: Primary Antibodies
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SKU: EB08850
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
lysophospholipase-like|MGL|MGLL|HU-K5|monoglyceride lipase
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_009214.1; NP_001003794.1
Blocking Peptide
EBP08850
Immunogen
Peptide with sequence C-QDLPHLVNADGQY, from the internal region (near N Terminus) of the protein sequence according to NP_009214.1; NP_001003794.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_009214.1; NP_001003794.1).
Peptide Sequence
C-QDLPHLVNADGQY
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human, Mouse
Human Gene ID
11343
Mouse Gene ID
23945
Rat Gene ID
29254
Product Grade
IHC Results
In paraffin embedded Human Liver, expression of MGLL is detected in hepatocytes. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 35kDa band observed in Mouse Adipose lysates and in preliminary testing of Human, Cerebellum lysate and in lysate of cell line NIH3T3 (calculated MW of 33.3kDa according to NP_001003794.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat for this particular batch.
Application Type
Pep-ELISA, WB, IHC, IF, FC

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