Goat Anti-E2F8 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB09086
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
E2F family member 8|FLJ23311|E2F transcription factor 8|E2F8
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of A431 and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_078956.2
Blocking Peptide
EBP09086
Immunogen
Peptide with sequence C-KSIESDRRKINSAPS , from the internal region of the protein sequence according to NP_078956.2.
Peptide Sequence
C-KSIESDRRKINSAPS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Cow
Reactive Species
Human
Human Gene ID
79733
Mouse Gene ID
108961
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary testing showed a band at approx 80kDa in Human K562 and Mouse Neuro2a cell lysate after 0.3µg/ml antibody staining. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IF, FC

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