Goat Anti-S100A9 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB09146
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
calgranulin B|S100 calcium-binding protein A9 (calgranulin B)|S100 calcium-binding protein A9|S100 calcium binding protein A9 (calgranulin B)|P14|NIF|MRP14|MIF|MAC387|LIAG|L1AG|CGLB|CFAG|CAGB|60B8AG|S100 calcium binding protein A9|S100A9
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of MCF7, U2OS and THP-1 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_002956.1
Blocking Peptide
EBP09146
Immunogen
Peptide with sequence C-DTNADKQLSFEEF , from the internal region of the protein sequence according to NP_002956.1.
Peptide Sequence
C-DTNADKQLSFEEF
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
6280
Product Grade
IHC Results
Paraffin embedded Human Lung. Recommended concentration: 2-4µg/ml. Paraffin embedded Human Spleen. Recommended concentration: 6-7µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Approx 13-14kDa band observed in Human Bone Marrow lysates and approx 14-15kDa in Human Gastrointestinal cancer lysates (calculated MW of 13.2kDa according to NP_002956.1). Recommended concentration: 0.5-2µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, IF, FC

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