Goat Anti-SATB1 Antibody

$464.00

Category: Primary Antibodies
SKU Unit Size Price

Select a unit size:

SKU: EB09448
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA\1s)|special AT-rich sequence binding protein 1|DNA-binding protein SATB1|SATB homeobox 1|SATB1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of Jurkat cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Accession ID
NP_002962.1; NP_001182399.1; NP_001309805.1
Blocking Peptide
EBP09448
Immunogen
Peptide with sequence C-ESDEENRQKTRPRT, from the internal region of the protein sequence according to NP_002962.1; NP_001182399.1; NP_001309805.1.
Peptide Sequence
C-ESDEENRQKTRPRT
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Cow
Reactive Species
Human
Human Gene ID
6304
Mouse Gene ID
20230
Rat Gene ID
316164
Product Grade
IHC Results
In paraffin embedded Human Thymus shows select nuclear staining. Recommended concentration: 3-5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx. 90kDa band observed in nuclear lysates of cell lines A431 and Jurkat and in lysates of cell lines U251 and Daudi (calculated MW of 89.0kDa according to NP_001182399.1). Recommended concentration: 0.1-0.5µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, IF, FC

Related products