Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names purinergic receptor P2X7|P2Z receptor|P2X7 receptor|P2X purinoceptor 7|ATP receptor|P2X7|MGC20089|purinergic receptor P2X, ligand-gated ion channel, 7|P2RX7 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the cytoplasm and plasma membranes of HeLa cells. Recommended concentration: 10µg/ml. This antibody has been successfully used in IF on Mouse: Thaler R et al. (2015) PMID: 25491310.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_002553.3 | |
Blocking Peptide EBP09509 | |
Immunogen Peptide with sequence YETNKVTRIQSMNY-C, from the N Terminus of the protein sequence according to NP_002553.3. | |
Peptide Sequence YETNKVTRIQSMNY-C | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog | |
Reactive Species Human, Mouse | |
Human Gene ID 5027 | |
Mouse Gene ID 18439 | |
Rat Gene ID 29665 | |
Product Grade  | |
IHC Results In paraffin embedded Human Brain Cortex shows membranous staining of cell bodies and processes. Recommended concentration: 3-6µg/ml. Paraffin embedded Human Kidney. Recommended concentration: 3.75µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:16000. | |
Western Blot Approx 37kDa and 70kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 68.6kDa according to NP_002553.2). Recommended concentration: 1-3µg/ml. Primary incubation was an 1 hour. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. | |
Application Type Pep-ELISA, WB, IHC, IF, FC |
Goat Anti-P2RX7 / P2X7 receptor Antibody
$464.00
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Selected References [{"pmid": 25491310, "intro": "This antibody has been successfully used in IF on Mouse:", "title": "Acute-phase protein serum amyloid A3 is a novel paracrine coupling factor that controls bone homeostasis.", "author": "Thaler R, Sturmlechner I, Spitzer S, Riester SM, Rumpler M, Zwerina J, Klaushofer K, van Wijnen AJ, Varga F.", "journal": "FASEB J. 2015 Apr;29(4):1344-59."}] |