Goat Anti-POU2AF1 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB10018
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
BOB1|OBF1|OBF-1|OCAB|POU class 2 associating factor 1|POU domain, class 2, associating factor 1|POU2AF1
Usage Summary
Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.
Accession ID
NP_006226.2
Blocking Peptide
EBP10018
Immunogen
Peptide with sequence C-DSDAYALNHTLSVE, from the C Terminus of the protein sequence according to NP_006226.2.
Peptide Sequence
C-DSDAYALNHTLSVE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Dog, Cow
Reactive Species
Human
Human Gene ID
5450
Product Grade
IHC Results
In paraffin embedded Human Spleen shows nuclear staining in the germinal center with stronger nucleoli staining in some of the B cells. Recommended concentration, 3-5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
In transfected HEK293 transiently expressing full-length Human POU2AF1 (myc and DYKDDDDK tagged), a band of approx. 38kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2µg/ml.
Application Type
Pep-ELISA, WB-Trf, IHC

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