Goat Anti-CRISP2 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

CRISP2|TSP1|TPX1|testis specific protein 1 (probe H4-1 p3-1)|OTTHUMP00000016589|MGC111136|glyceraldehyde-3-phosphate dehydrogenase-like 5|GAPDL5|cysteine-rich secretory protein 2|CRISP-2

NP_003287.1

EBP09849

Peptide with sequence C-QHSDPEDRKTSTR, from the internal region of the protein sequence according to NP_003287.1.

Reported variants represent identical protein: NP_003287.1, NP_001135879.1, NP_001135889.1, NP_001135907.1, NP_001135880.1.

C-QHSDPEDRKTSTR

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Pig, Dog

Human

7180

Paraffin embedded Human Testis. Recommended concentration: 3.75µg/ml.

Antibody detection limit dilution 1:8000.

In transfected HEK293 transiently expressing Human CRISP2 (MYC and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration: 0.5-1µg/ml. Primary incubations were for 1 hour. Preliminary experiments gave an approx 55kDa band in Human Testis lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 27.3kDa according to NP_003287.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide.

Pep-ELISA, WB-Trf, IHC