Goat Anti-IL1R2 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB10107
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
IL1R2|type II interleukin-1 receptor, beta|MGC47725|interleukin 1 receptor, type II|IL1RB|CD121b|antigen CDw121b
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of A549 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_004624.1; NP_001248348.1
Blocking Peptide
EBP10107
Immunogen
Peptide with sequence C-QYNITRSIELRIKK, from the internal region of the protein sequence according to NP_004624.1; NP_001248348.1.
Product Comments
This antibody is expected to cross-react with both reported isoforms (NP_004624.1; NP_001248348.1). Reported variants represent identical protein: NP_775465.1, NP_004624.1
Peptide Sequence
C-QYNITRSIELRIKK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
7850
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 45kDa band observed in lysates of cell lines LNCaP and MOLT-4 (calculated MW o 45.4kDa according to NP_004624.1). Recommended concentration:0.3-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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