Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names PLA2G4A|PLA2G4|phospholipase A2, group IVA (cytosolic, calcium-dependent)|phosphatidylcholine 2-acylhydrolase|MGC126350|lysophospholipase|cytosolic phospholipase A2, group IVA|cPLA2-alpha|calcium-dependent phospholipid-binding protein | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A549 cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_077734.1 | |
Blocking Peptide EBP10113 | |
Immunogen Peptide with sequence C-QEKTFRQQRKEHIR, from the internal region of the protein sequence according to NP_077734.1. | |
Peptide Sequence C-QEKTFRQQRKEHIR | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat | |
Reactive Species Human | |
Human Gene ID 5321 | |
Product Grade  | |
IHC Results Paraffin embedded Human Uterus. Recommended concentration: 3.75µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Approx 80-85kDa band observed in lysates of cell line A549 (calculated MW of 85.2kDa according to NP_077734.1). Preliminary experiments also showed an 85- 90kDa band in lysates of cell lines A431, HeLa and NIH-3T3 Recommended concentration:0.3-1µg/ml. Primary incubation 1 hour at room temperature.Preliminary testing was unsuccessful on Rat for this particular batch. | |
Application Type Pep-ELISA, WB, IF, FC, IHC |
Goat Anti-PLA2G4A Antibody
$464.00
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