Goat Anti-FANCF (aa217-228) Antibody

$423.00

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SKU: EB10140
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
FANCF|MGC126856|Fanconi anemia, complementation group F|FAF
Accession ID
NP_073562.1
Blocking Peptide
EBP10140
Immunogen
Peptide with sequence C-RPQEELEPGIHK, from the internal region of the protein sequence according to NP_073562.1.
Peptide Sequence
C-RPQEELEPGIHK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
2188
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
In transfected HEK293 transiently expressing full-length Human FANCF (myc and DYKDDDDK tagged), a band of approx. 40kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.2-1µg/ml.
Application Type
Pep-ELISA, WB-Trf
Selected References
[{"pmid": 32075772, "intro": "This antibody has been successfully used in the following paper:", "title": "Cooperation of the ATM and Fanconi Anemia/BRCA Pathways in Double-Strand Break End Resection.", "author": "Mu-Yan Cai, Connor E. Dunn, Wenxu Chen, Bose S. Kochupurakkal, Huy Nguyen, Lisa A. Moreau, Geoffrey I. Shapiro, Kalindi Parmar, David Kozono, and Alan D. D’Andrea", "journal": "Cell Rep. 2020 Feb 18; 30(7): 2402-2415.e5."}]