Goat Anti-BOK Antibody

$423.00

Category: Primary Antibodies
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SKU: EB10361
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
BOK|MGC4631|BOKL|BCL2-related ovarian killer|BCL2L9
Accession ID
NP_115904.1
Blocking Peptide
EBP10361
Immunogen
Peptide with sequence C-HISLQSEPVVTD, from the internal region of the protein sequence according to NP_115904.1.
Peptide Sequence
C-HISLQSEPVVTD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Cow
Reactive Species
Human
Human Gene ID
666
Mouse Gene ID
51800
Product Grade
IHC Results
Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Preliminary experiments gave bands at approx 55kDa and 20kDa in Human Testis lysates after 1µg/ml antibody staining, and 17kDa in Mouse Spleen and Thymus lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 23.3kDa according to NP_115904.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Application Type
Pep-ELISA, IHC

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