Goat Anti-PIM2 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB10834
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
serine/threonine-protein kinase pim-2| serine/threonine protein kinase pim-2| proto-oncogene Pim-2 (serine threonine kinase)| pim-2h| pim-2 oncogene|PIM2
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_006866.2
Blocking Peptide
EBP10834
Immunogen
Peptide with sequence C-QTPAEDVPLNPSK, from the C Terminus of the protein sequence according to NP_006866.2.
Peptide Sequence
C-QTPAEDVPLNPSK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
11040
Product Grade
IHC Results
Paraffin embedded Human Testis. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx. 30kDa band observed in lysates of cell line K562 and in preliminary testing of cell lines HeLa and Caco-2 and approx. 30-35kDa observed in lysates of cell line HepG2 (calculated MW of 34.2kDa according to NP_006866.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, IF, FC

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