Goat Anti-TERT (aa597-611) Antibody

$423.00

Category: Primary Antibodies
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SKU: EB11292
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
TERT| TRT| TP2| telomerase-associated protein 2| telomerase reverse transcriptase| telomerase catalytic subunit| TCS1| hEST2| EST2
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_937983.2; NP_001180305.1
Blocking Peptide
EBP11292
Immunogen
Peptide with sequence C-QLRELSEAEVRQHRE, from the internal region of the protein sequence according to NP_937983.2; NP_001180305.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_937983.2; (NP_001180305.1).
Peptide Sequence
C-QLRELSEAEVRQHRE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
7015
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary testing showed a band at approx 125kDa in lysates of cell lines A549 and CaCo-2 after 1µg/ml antibody staining (calculated MW of 127kDa according to NP_937983.2). Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IF, FC

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