Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names Hdac2| Yy1bp| YY1 transcription factor-binding protein| YAF1| OTTMUSP00000022803| mRPD3| histone deacetylase 2| HD2| D10Wsu179e | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the nuclei of U251 cells and additionally in the membrane and cytoplasm of NIH3T3 cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_032255.2 | |
Blocking Peptide EBP11448 | |
Immunogen Peptide with sequence C-PEDAVHEDSGDE, from the internal region of the protein sequence according to NP_032255.2. | |
Product Comments This antibody imay cross-react with HDAC1 | |
Peptide Sequence C-PEDAVHEDSGDE | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Pig, Cow | |
Reactive Species Human, Mouse | |
Human Gene ID 3066 | |
Mouse Gene ID 15182 | |
Rat Gene ID 84577 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:2000. | |
Western Blot Approx 55-60kDa band observed in nuclear lysates of cell line HEK293 (calculated MW of 55.3kDa according to NP_032255.2). Recommended concentration: 1-2µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IF, FC |
Goat Anti-Hdac2 (mouse) Antibody
$464.00
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