Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size 100 µg
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names RP23-415G1.3\|pp160\|phosphoprotein of 160 kDa\|IRS-4\|Irs4\|insulin receptor substrate 4
Usage Summary Immunofluorescence: This product has been successfully used in IF on Mouse: Harriet M Jackson et al, BMC Genomics. 2013 Nov 25;14:831. PMID: 24274089.
Accession ID NP_034702.2
Blocking Peptide EBP11828
Immunogen Peptide with sequence C-DRGTKERKEAKEVRD, from the internal region of the protein sequence according to NP_034702.2.
Peptide Sequence C-DRGTKERKEAKEVRD
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions Refrigerated
Predicted Species Mouse, Rat
Reactive Species Mouse
Mouse Gene ID 16370
Rat Gene ID 315350
Product Grade
ELISA Detection Limit Antibody detection limit dilution 1:128000.
Western Blot Approx 150kDa band observed in Mouse Heart lysates (calculated MW of 130kDa according to NP_034702.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreyer et al, jEndocrinology. 2003 Apr;144(4):1211-8. PMID: 12639902). Recommended concentration: 0.2-0.6µg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at ~39kDa. This band was successfully blocked by incubation with the immunizing peptide. This product has been successfully used in WB on Mouse: Ikink GJ et al, Nat Commun. 2016 Nov 23;7:13567. PMID: 27876799.
Application Type Pep-ELISA, WB, IF

Selected References

[{"pmid": 24274089, "intro": "This antibody has been successfully used in IF on Mouse:", "title": "Clustering of transcriptional profiles identifies changes to insulin signaling as an early event in a mouse model of Alzheimer’s disease", "author": "Harriet M Jackson, Ileana Soto, Leah C Graham, Gregory W Carter and Gareth R Howel.", "journal": "BMC Genomics. 2013 Nov 25;14:831. "}, {"pmid": 27876799, "intro": "This antibody has been successfully used in Western blot on Mouse:", "title": "IRS4 induces mammary tumorigenesis and confers resistance to HER2-targeted therapy through constitutive PI3K/AKT-pathway hyperactivation", "author": "Ikink GJ, Boer M, Bakker ER, Hilkens J", "journal": "Nat Commun. 2016 Nov 23;7:13567"}]

Goat Anti-Irs4 (mouse aa633-647) Antibody

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