Goat Anti-MK5 / MAPKAPK5 (aa192-206) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

p38-regulated/activated protein kinase|MAPKAPK-5|MAPKAP kinase 5|MAPK-activated protein kinase 5|MAP kinase-activated protein kinase 5|PRAK|MK5|MK-5|MAPKAP-K5|mitogen-activated protein kinase-activated protein kinase 5|MAPKAPK5

Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5ug EB11927 or EB11928. Western blots of the IP were labelled with mouse anti-MK5 (SC) or with rabbit anti-ERK3 serum (CST). An approx 55kDa of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

NP_003659.2; NP_620777.1

EBP11928

Peptide with sequence C-PQVLEAQRRHQKEKS, from the internal region of the protein sequence according to NP_003659.2; NP_620777.1.

C-PQVLEAQRRHQKEKS

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Pig, Cow

Human, Mouse

8550

17165

498183

In paraffin embedded Human Kidney shows nuclear staining in the DCT. Recommended concentration, 4-8µg/ml.

Antibody detection limit dilution 1:64000.

In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP , only the expected band was observed in the MK5 lane after EB11928 labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration, 0.5-1µg/ml.

Pep-ELISA, WB-Trf, IP, IHC