Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names troponin I, cardiac muscle|cTnI|TNNC1|RCM1|CMH7|CMD2A|CMD1FF|troponin I type 3 (cardiac)|TNNI3 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. Recommended concentration: 10µg/ml. | |
Accession ID NP_000354.4 | |
Blocking Peptide EBP11958 | |
Immunogen Peptide with sequence C-KVTKNITEIAD, from the internal region of the protein sequence according to NP_000354.4. | |
Peptide Sequence C-KVTKNITEIAD | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Pig, Cow | |
Reactive Species Human, Mouse, Rat, Pig | |
Human Gene ID 7137 | |
Mouse Gene ID 21954 | |
Rat Gene ID 29248 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx 25+26kDa doublet band observed in Human Heart, approx 24+27kDa in Mouse Heart, approx 25kDa in Rat Heart and approx 27kDa in Pig Heart lysates (calculated MW of 24kDa according to Human NP_000354.4, and Pig NP_001092069.1 and 24.3kDa according to Mouse NP_033432.1 and Ra tNP_058840.1 ). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IF |
Goat Anti-TNNI3 (aa117-127) Antibody
$423.00
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