Goat Anti-ATF6 (aa437-449) Antibody

$464.00

Category: Primary Antibodies
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SKU: EB12080
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
cyclic AMP-dependent transcription factor ATF-6 alpha|cAMP-dependent transcription factor ATF-6 alpha|ATF6A|activating transcription factor 6|ATF6
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_031374.2
Blocking Peptide
EBP12080
Immunogen
Peptide with sequence C-NSYRYDHSVSNDK, from the internal region of the protein sequence according to NP_031374.2.
Peptide Sequence
C-NSYRYDHSVSNDK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Rat, Dog, Pig, Cow
Reactive Species
Human
Human Gene ID
22926
Rat Gene ID
304962
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 75-80kDa band observed in lysates of cell lines HeLa and MCF7, and in HeLa and Jurkat nuclear cell lysates (calculated MW of 74.6kDa according to NP_031374.2). Recommended concentration: 0.3-2µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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