Goat Anti-IFNGR1 (aa181-193) Antibody

$464.00

Category: Primary Antibodies
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SKU: EB12101
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
interferon-gamma receptor alpha chain|immune interferon receptor 1|antiviral protein, type 2|IFN-gamma-R1|IFN-gamma receptor 1|CDw119|CD119 antigen|AVP, type 2|IFNGR|CD119|interferon gamma receptor 1|IFNGR1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the membranes of Caco-2 and THP-1 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_000407.1; NP_001350455.1; NP_001350456.1
Blocking Peptide
EBP12101
Immunogen
Peptide with sequence C-SEIQYKILTQKED, from the internal region of the protein sequence according to NP_000407.1; NP_001350455.1; NP_001350456.1.
Peptide Sequence
C-SEIQYKILTQKED
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
3459
Product Grade
IHC Results
Paraffin embedded Human Lung. Recommended concentration: 4-6µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx. 70kDa band observed in lysates of cell lines K562, Caco-2 and HepG2, and approx. 50kDa in Human Spleen lysates (calculated MW of 50.1kDa according to NP_001350456.1). The 70kDa observed molecular weight corresponds to the glycosylated form. Recommended concentration: 1-2µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, IHC, FC

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