Goat Anti-NPHS2 / SRN1 Antibody

$423.00

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SKU: EB12149
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
podocin|SRN1|PDCN|nephrosis 2, idiopathic, steroid-resistant (podocin)|NPHS2
Accession ID
NP_055440.1
Blocking Peptide
EBP12149
Immunogen
Peptide with sequence C-SPSKPVEPLNPKK, from the C Terminus of the protein sequence according to NP_055440.1.
Peptide Sequence
C-SPSKPVEPLNPKK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Pig, Cow
Reactive Species
Human, Mouse
Human Gene ID
7827
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx 42-45kDa band observed in Human and Mouse Kidney lysates (calculated MW of 42.2kDa according to Human NP_055440.1 and Mouse NP_569723.1). Recommended concentration: 0.5-2µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB
Selected References
[{"pmid": 34440683, "intro": "This antibody (previous batch) has been successfully used in IF on Mouse:", "title": "Novel Human Podocyte Cell Model Carrying G2/G2 APOL1 High-Risk Genotype", "author": "Pepe M. Ekulu et al.", "journal": "Cells. 2021 Aug; 10(8): 1914."}]