Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names wegener autoantigen|serine proteinase, neutrophil|neutrophil proteinase 4|myeloblastin|leukocyte proteinase 3|azurophil granule protein 7|Wegener granulomatosis autoantigen|NP-4|C-ANCA antigen|PR3|PR-3|P29|NP4|MBT|MBN|CANCA|C-ANCA|AGP7|ACPA|proteinase 3|PRTN3 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_002768.3 | |
Blocking Peptide EBP12324 | |
Immunogen Peptide with sequence C-HNVRTQEPTQQ, from the internal region of the protein sequence according to NP_002768.3. | |
Peptide Sequence C-HNVRTQEPTQQ | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 5657 | |
Product Grade  | |
IHC Results Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:8000. | |
Western Blot Approx. 27kDa band observed in Human Spleen lysates, approx. 25kDa in Human Bone Marrow lysates, and approx. 26kDa in preliminary testing of THP-1 cell lysate (calculated MW of 27.8kDa according to NP_002768.3). Recommended concentration:0.3-1µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IHC, IF, FC |
Goat Anti-proteinase 3 / myeloblastin (aa88-98) Antibody
$464.00
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