Goat Anti-AIFM1 (aa183-195) Antibody

$464.00

Category: Primary Antibodies
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SKU: EB12621
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
striatal apoptosis-inducing factor|programmed cell death 8 (apoptosis-inducing factor)|apoptosis-inducing factor 1, mitochondrial|PDCD8|COXPD6|COWCK|CMTX4|AIF|apoptosis-inducing factor, mitochondrion-associated, 1|AIFM1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa and NIH3T3 cells. Recommended concentration: 5-10µg/ml.
Accession ID
NP_004199.1; NP_665811.1; NP_001124319.1
Blocking Peptide
EBP12621
Immunogen
Peptide with sequence C-DDPNVTKTLRFKQ, from the internal region of the protein sequence according to NP_004199.1; NP_665811.1; NP_001124319.1.
Product Comments
This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1) and isoform 5 (NP_001124319).
Peptide Sequence
C-DDPNVTKTLRFKQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Cow
Reactive Species
Human, Mouse
Human Gene ID
9131
Product Grade
IHC Results
Paraffin embedded Human Kidney. Recommended concentration: 5-7µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 70kDa band observed in lysates of cell line Jurkat (calculated MW of 66.9kDa according to NP_004199.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, IHC

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