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Code Name Applications Availability Size Tested species Price Grade  
EB05393 Goat Anti-AIBZIP / CREB3L4 Antibody Pep-ELISA, WB, IHC, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

Ordering Information for

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our US partner Abcore LLC.

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05393 1

EB05393 optimised QC. Primary incubation 1 hour at room temperature.

Images A, B: MDA-MB-231, A431 cell lysate at primary Ab concentration 0.1µg/ml, Image C: MCF7 cell lysate at primary Ab concentration 0.003µg/ml. (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
EB05393 2

EB05393 optimised QC. Primary incubation 1 hour at room temperature.

Images A, B: Human Kidney and Placenta lysate at primary Ab concentration 0.5µg/ml. (Loaded 35µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
EB05393 3
EB05393 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear, nuclear membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05393 4
EB05393 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05393 5
EB05393 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB05393 6
EB05393 (2.5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: AIBZIP, cAMP responsive element binding protein 3-like 4, JAL, hJAL, ATCE1, CREB3, CREB4, androgen-induced basic leucine zipper, RP11-422P24.8, OTTHUMP00000035266, cAMP responsive element binding protein 1
Official Symbol: CREB3L4
Accession Number(s): NP_570968.1; NP_001242909.1
Human GeneID(s): 148327
Important Comments: This antibody is expected to recognize both reported isoforms (NP_570968.1; NP_001242909.1). Reported variants represent identical protein: NP_001242908.1, NP_570968.1, NP_001242907.1 Reported variants represent identical protein: NP_001242909.1, NP_001242910.1

Immunogen

Peptide with sequence C-PSGRIRSVLHADEM, from the C Terminus of the protein sequence according to NP_570968.1; NP_001242909.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 50kDa band observed in Human Kidney and Placenta lysate, and in lysates of cell lines MDA-MB-231, A431 and MCF7 (calculated MW of 43.4kDa according to NP_570968.1). Recommended concentration: 0.003-0.5µg/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Prostate shows strong staining of cytoplasm in the secretory cells of the gland. Recommended concentration: 2-4µg/ml.

Immunofluorescence: Strong expression of the protein seen in A431 and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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