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Goat Anti-Alpha2-antiplasmin Antibody (EB08777)

Related Products: EBP08777 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08777 Goat Anti-Alpha2-antiplasmin Antibody Pep-ELISA, WB, IHC, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08777 1
EB08777 (0.1µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB08777 2
EB08777 (3.8µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB08777 3
EB08777 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB08777 4
EB08777 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: SERPINF2, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2, A2AP, AAP, ALPHA-2-PI, API, PLI, alpha-2-plasmin inhibitor, serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
Official Symbol: SERPINF2
Accession Number(s): NP_000925.2; NP_001159393.1
Human GeneID(s): 5345
Important Comments: This antibody is expected to recognise both reported isoforms (NP_000925.2; NP_001159393.1). Reported variants represent identical protein (NP_000925.2; NP_001159392.1).

Immunogen

Peptide with sequence C-KDFLQSLKGFPRGDK, from the internal region of the protein sequence according to NP_000925.2; NP_001159393.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 70kDa band observed in lysates of cell line HepG2 (calculated MW of 54.6kDa according to NP_000925.2). The observed molecular weight is observed by other sources. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. This antibody has been successfully used in WB on Human: Abdul S et al. (2020) PMID: 32034861.
IHC: In paraffin embedded Human Liver shows spotty cytoplasm staining in hepatocytes. Recommended concentration: 3-5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific Reference

This antibody has been successfully used in Western blot on Human:
Abdul S, Dekkers DHW, Ariëns RAS, Leebeek FWG, Rijken DC, Uitte de Willige S
On the localization of the cleavage site in human alpha-2-antiplasmin, involved in the generation of the non-plasminogen binding form.
J Thromb Haemost. 2020 Feb 8
PMID: 32034861

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