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Goat Anti-AMHR2 (aa88-99) Antibody (EB12625)

Related Products: EBP12625 - Immunizing Peptide, EBP12626 - Immunizing Peptide, Goat Anti-AMHR2 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB12625 Goat Anti-AMHR2 (aa88-99) Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB12625 1
EB12625 (1µg/ml) staining of MCF7 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12625 2
EB12625 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB12625 3
EB12625 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: AMHR2, anti-Mullerian hormone receptor, type II, AMHR, MISR2, MISRII, MRII, AMH type II receptor, MIS type II receptor, Muellerian inhibiting substance type II receptor, Mullerian inhibiting substance type II receptor, anti-Muellerian hormone type II receptor
Official Symbol: AMHR2
Accession Number(s): NP_065434.1; NP_001158162.1; NP_001158163.1
Human GeneID(s): 269
Important Comments: This antibody is expected to recognize all reported isoforms (NP_065434.1; NP_001158162.1; NP_001158163.1).

Immunogen

Peptide with sequence ESLHCDPSPRAH, from the internal region of the protein sequence according to NP_065434.1; NP_001158162.1; NP_001158163.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx. 60+65kDa bands were observed in lysates of cell line MCF7 (calculated MW of 62.7kDa according to NP_065434.1). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in MCF7 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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