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Goat Anti-APOL3 Antibody (EB08917)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB08917 | Goat Anti-APOL3 Antibody | Pep-ELISA, WB |  |
100µg specific antibody in 200µl | Human | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. |
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EB08917 (1µg/ml) staining of DAUDI lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Target Protein
Principal Names: APOL3, apolipoprotein L, 3, APOLIII, CG12-1, OTTHUMP00000028913, TNF-inducible protein CG12-1, apolipoprotein L-III, apolipoprotein L3Official Symbol: APOL3
Accession Number(s): NP_663615.1, NP_055164.1, NP_663616.1
Human GeneID(s): 80833
Important Comments: This antibody is expected to recognize isoform 1 (NP_663615.1), isoform 2 (NP_055164.1, NP_085147.1, NP_663614.1) and isoform 3 (NP_663616.1, NP_663617.1).
Immunogen
Peptide with sequence ELTQIYQRLNPCHTH, from the C Terminus of the protein sequence according to NP_663615.1, NP_055164.1, NP_663616.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:1000.Western blot: Approx. 40kDa band observed in lysates of cell lines DAUDI and MOLT4 (calculated MW of 44.3kDa according to NP_663615.1). Recommended concentration: 1-3µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human