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Goat Anti-ARHGEF4 Antibody (EB06107)

Related Products: EBP06107 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06107 Goat Anti-ARHGEF4 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06107 1
EB06107 (2µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB06107 2
HEK293 overexpressing ARHGEF4 (RC215591) and probed with EB06107 (mock transfection in first lane), tested by Origene.
Click here to download PDF datasheet.

Target Protein

Principal Names: ARHGEF4, Rho guanine nucleotide exchange factor (GEF) 4, ASEF, GEF4, STM6, KIAA1112, DKFZp434G2016, APC-stimulated guanine nucleotide exchange factor, ASEF1, Rho guanine nucleotide exchange factor 4
Official Symbol: ARHGEF4
Accession Number(s): NP_056135.2; NP_127462.1
Human GeneID(s): 50649
Important Comments: This antibody is expected to recognise both reported isoforms (NP_056135.2; NP_127462.1).

Immunogen

Peptide with sequence PWEEPAGEKPSCS, from the N Terminus of the protein sequence according to NP_056135.2; NP_127462.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 80kDa band observed in Human Brain lysates and in transfected HEK293 transiently expressing ARHGEF4 (calculated MW of 79.1kDa according to NP_056135.2 and 76.4kDa according to 127462.1). Recommended concentration: 2-4µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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