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Goat Anti-ARP2/3 subunit 1B Antibody (EB05435)

Related Products: EBP05435 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05435 Goat Anti-ARP2/3 subunit 1B Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05435 1
EB05435 staining (2µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
EB05435 2
EB05435 (5µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ARPC1B, actin related protein 2/3 complex, subunit 1B, 41kDa, ARC41, p40-ARC, p41-ARC, ARP2/3 protein complex subunit p41, actin related protein 2/3 complex, subunit 1A (41 kD), OTTHUMP00000206020
Official Symbol: ARPC1B
Accession Number(s): NP_005711.1
Human GeneID(s): 10095

Immunogen

Peptide with sequence AYHSFLVEPISCH, from the N Terminus of the protein sequence according to NP_005711.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 37kDa band observed in Human Liver lysates (calculated MW of 40.9kDa according to NP_005711). Recommended concentration: 2-4µg/ml. A minor band of unknown identity was also consistently observed at 28kDa.
IHC: Paraffin embedded Human Thymus. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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