Choose your country here (United States of America)
Home

Goat Anti-ASRGL1 / ALP Antibody (EB08138)

Code Name Applications Availability Size Tested species Price Grade  
EB08138 Goat Anti-ASRGL1 / ALP Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

Ordering Information for United States of America (change)

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

EB08138 1
EB08138 (0.5µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ASRGL1, ALP, asparaginase like 1, ALP1, FLJ22316, asparaginase-like 1 protein
Official Symbol: ASRGL1
Accession Number(s): NP_001077395.1; NP_079356.3
Human GeneID(s): 80150
Important Comments: Both reported variants represent identical protein (NP_001077395.1 and NP_079356.3).

Immunogen

Peptide with sequence EKHEKGAQKTDCQ, from the internal region of the protein sequence according to NP_001077395.1; NP_079356.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 34kDa band observed in Human Tosil and lysteof cell line Hela lysates (calculated MW of 32.1kDa according to NP_001077395.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human