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Goat Anti-ASRGL1 / ALP Antibody (EB08138)

Related Products: EBP08138 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08138 Goat Anti-ASRGL1 / ALP Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08138 1
EB08138 (0.5µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ASRGL1, ALP, asparaginase like 1, ALP1, FLJ22316, asparaginase-like 1 protein
Official Symbol: ASRGL1
Accession Number(s): NP_001077395.1; NP_079356.3
Human GeneID(s): 80150
Important Comments: Both reported variants represent identical protein (NP_001077395.1 and NP_079356.3).

Immunogen

Peptide with sequence EKHEKGAQKTDCQ, from the internal region of the protein sequence according to NP_001077395.1; NP_079356.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 34kDa band observed in Human Tosil and lysteof cell line Hela lysates (calculated MW of 32.1kDa according to NP_001077395.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 20kDa. This band was successfully blocked by incubation with the immunizing peptide.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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