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Goat Anti-ATP1B1 Antibody (EB09640)

Related Products: EBP09640 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09640 Goat Anti-ATP1B1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09640 1
EB09640 (0.1µg/ml) staining of Rat Retina lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09640 2
EB09640 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ATP1B1, ATPase, Na+/K+ transporting, beta 1 polypeptide, ATP1B, MGC1798, Beta 1-subunit of Na(+),K(+)-ATPase, Na+/K+ -ATPase beta 1 subunit, Na, K-ATPase beta-1 polypeptide, Na,K-ATPase beta 1 subunit, OTTHUMP00000032537, OTTHUMP00000032538, adenosinetriphosphatase, sodium/potassium-dependent ATPase beta-1 subunit, sodium/potassium-transporting ATPase beta-1 chain
Official Symbol: ATP1B1
Accession Number(s): NP_001668.1; NP_001001787.1
Human GeneID(s): 481
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001668.1; NP_001001787.1).

Immunogen

Peptide with sequence C-KTEISFRPNDPKSYE, from the internal region of the protein sequence according to NP_001668.1; NP_001001787.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 48kDa band observed in Rat Retina lysates and 50kDa in Mouse and Rat Kidney lysates (calculated MW of 35.1kDa according to Human NP_001668.1, 35.2kDa according to Mouse NP_033851.1 and Rat NP_037245.2).The observed molecular weight corresponds to the glycosylated form (PMID: 20130120). Recommended concentration: 0.1-0.3µg/ml.
IHC: Paraffin embedded Human Small Intestine. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig

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