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Goat Anti-calbindin D28 Antibody (EB11759)

Related Products: EBP11759 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB11759 Goat Anti-calbindin D28 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11759 1
EB11759 (0.3µg/ml) staining of Human Kidney lysate, (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11759 2
EB11759 (0.3µg/ml) staining of Rat Brain (A) and (0.1µg/ml) Kidney (B) lysate, (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11759 3
EB11759 (5µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB11759 4
EB11759 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: CALB, CALB1, calbindin, calbindin 1, (28kD), calbindin 1, 28kDa, calbindin D28, D-28K, OTTHUMP00000166027, OTTHUMP00000225441, RTVL-H protein, vitamin D-dependent calcium-binding protein, avian-type
Official Symbol: CALB1
Accession Number(s): NP_004920.1
Human GeneID(s): 793
Non-Human GeneID(s): 83839 (rat)
Important Comments: Immunizing peptide overlaps EF hand 2, but not any calcium binding site.

Immunogen

Peptide with sequence C-KTFVDQYGQRDDGK, from the internal region of the protein sequence according to NP_004920.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx. 26kDa band observed in Human and Rat Kidney lysates and in Rat Brain lysates (calculated MW of 30.0kDa according to Human NP_004920.1and Rat NP_114190.1). The observed molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5-7µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Rat, Dog, Pig, Cow

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